Inicio
En el grupo de Metaloproteinas y Sistemas Modelo estudiamos metaloproteinas que participan en diferentes vías de algunos ciclos biogeoquímicos (i.e. nitrógeno, azufre y carbono) y que contienen metales de transición como el cobre, hierro o molibdeno. Además sintetizamos complejos de metales de transición paramagnéticos que reproducen las propiedades estructurales y/o magnéticas de sitios activos de metaloenzimas y estudiamos sus propiedades magnéticas, electrónicas y fisicoquímicas.
En nuestro laboratorio contamos con las técnicas necesarias para realizar la caracterización bioquímica (masa molecular de monómeros y oligómeros, contenido de metales/cofactores), cinética (estado estacionario), electroquímica (Protein Film Voltammetry) y espectroscópica (UV-visible y Resonancia Paramagnética Electrónica) de metaloenzimas y compuestos organometálicos.
Equipo de Trabajo
Investigadores
Dr. Carlos D. Brondino (Jefe de Grupo, Investigador Principal CIC-CONICET)
Dr. Pablo J. González (Investigador Adjunto CIC-CONICET)
Dra. María Gabriela Rivas (Investigador Adjunto CIC-CONICET)
Dr. Félix M. Ferroni (Investigador Asistente CIC-CONICET)
Estudiantes de Posdoctorado
Estudiantes de Doctorado
Estudiantes de Grado
Emilia Hintermeister (Pasante 2017-2018, Tesis de grado Lic. en Biotecnología 2019)
Hilen C. Elizalde (Cientibeca 2017-2018, Pasantía 2019-2020)
Sofía Garmendia (Pasante 2017-2018, Tesis de grado Lic. en Biotecnología 2019)
Florencia Schmidt (Pasante 2017-2018, Cientibeca y Tesis de grado Lic. en Biotecnología 2018-2019)
Miembros anteriores
Verónica Vesco (Pasante 2016)
Victoria Slaboch (Pasante 2016)
María Florencia Rojas (Pasante 2015)
Sara Charadia (Pasante 2016)
Emilia Cicazzo (Cientibeca 2017-2018)
Lic. Cintia Ramirez (Pasante 2017-2018, Tesis de grado Lic. en Biotecnología 2019)
Financiamiento
El grupo de Metaloproteínas y Sistemas Modelo cuenta con financiación de la Agencia Nacional de Promoción Científica y Tecnológica (ANPCYT), el CONICET y la Universidad Nacional del Litoral.
Publicaciones
2015
Ligand Binding to Chlorite Dismutase from Magnetospirillum sp. De Schutter A, Correia HD, Freire DM, Rivas MG, Rizzi A, Santos-Silva T, González PJ, Van Doorslaer S. J Phys Chem-B 2015. En prensa.
Periplasmic Nitrate Reductase and Formate Dehydrogenase: Similar Molecular Architectures with Very Different Enzymatic Activities. Cerqueira NMFSA, Gonzalez PJ, Fernandes PA, Moura JJG, Ramos MJ. Acc. Chem. Res. 2015. En prensa.
Suarez, S.A., Neuman, N.I., Muñoz, M., Alvarez, L., Bikiel, D.E., Brondino, C.D., Ivanovic-Burmazovic, I., Miljkovic, J.L., Filipovic, M.R., Marti, M.A., Doctorovich, F. Nitric Oxide Is Reduced to HNO by Proton-Coupled Nucleophilic Attack by Ascorbate, Tyrosine, and Other Alcohols. A New Route to HNO in Biological Media? (2015) Journal of the American Chemical Society, 137 (14), pp. 4720-4727.
Gomez, M.C., Neuman, N.I., Dalosto, S.D., Gonzalez, P.J., Moura, J.J.G., Rizzi, A.C., Brondino, C.D. Isotropic exchange interaction between Mo and the proximal FeS center in the xanthine oxidase family member aldehyde oxidoreductase from Desulfovibrio gigas on native and polyalcohol inhibited samples: An EPR and QM/MM study (2015) Journal of Biological Inorganic Chemistry, 20 (2), pp. 233-242.
Correia, H.D., Marangon, J., Brondino, C.D., Moura, J.J.G., Romao, M.J., Gonzalez, P.J., Santos-Silva, T. Aromatic aldehydes at the active site of aldehyde oxidoreductase from Desulfovibrio gigas: Reactivity and molecular details of the enzyme-substrate and enzyme-product interaction (2015) Journal of Biological Inorganic Chemistry, 20 (2), pp. 219-229.
Neuman, N. I., Burna,E., Baggio,R.,Passeggi,M. C. G., Rizzi, A. C.,Brondino C. D. Transition from isolated to interacting copper(II) pairs in extended lattices evaluated by single crystal EPR spectroscopy (2015)Inorganic Chemistry Frontiers2, pp. 837-845.
Nair, R.R., Silveira, C.M., Diniz, M.S., Almeida, M.G., Moura, J.J.G., Rivas, M.G. Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess (2015) Journal of Biological Inorganic Chemistry, 20 (2), pp. 311-322.
Hamer, M., Suarez, S.A., Neuman, N.I., Alvarez, L., Munoz, M., Marti, M.A., Doctorovich, F., Discussing Endogenous NO/HNO Interconversion Aided by Phenolic Drugs and Vitamins, (2015) Inorganic Chemistry, 54 (19), pp. 9342-9350.
Freire, D.M., Rivas, M.G., Dias, A.M., Lopes, A.T., Costa, C., Santos-Silva, T., Van Doorslaer, S., Gonzalez, P.J. The homopentameric chlorite dismutase from Magnetospirillum sp. (2015) Journal of Inorganic Biochemistry, 151, pp. 1-9.
Moura, J.J.G., Bernhardt, P.V., Maia, L.B., Gonzalez, P.J. Molybdenum and tungsten enzymes: From biology to chemistry and back (2015) Journal of Biological Inorganic Chemistry, 20 (2), pp. 181-182.
2014
Maiti, B.K., Maia, L.B., Pal, K., Pakhira, B., Aviles, T., Moura, I., Pauleta, S.R., Nuñez, J.L., Rizzi, A.C., Brondino, C.D., Sarkar, S., Moura, J.J.G. One electron reduced square planar bis(benzene-1,2-dithiolato) copper dianionic complex and redox switch by O2/HO- (2014) Inorganic Chemistry, 53 (24), pp. 12799-12808.
Russo, M.G., Vega Hissi, E.G., Rizzi, A.C., Brondino, C.D., Salinas Ibanez, A.G., Vega, A.E., Silva, H.J., Mercader, R., Narda, G.E. Synthesis, physicochemical characterization, DFT calculation and biological activities of Fe(III) and Co(II)-omeprazole complexes. Potential application in the Helicobacter pylori eradication (2014) Journal of Molecular Structure, 1061 (1), pp. 5-13.
Neuman, N.I., Winkler, E., Peña, O., Passeggi, M.C.G., Rizzi, A.C., Brondino, C.D. Magnetic properties of weakly exchange-coupled high spin Co(II) ions in pseudooctahedral coordination evaluated by single crystal X-band EPR spectroscopy and magnetic measurements (2014) Inorganic Chemistry, 53 (5), pp. 2535-2544.
Ferroni, F.M., Marangon, J., Neuman, N.I., Cristaldi, J.C., Brambilla, S.M., Guerrero, S.A., Rivas, M.G., Rizzi, A.C., Brondino, C.D. Pseudoazurin from Sinorhizobium meliloti as an electron donor to copper-containing nitrite reductase: Influence of the redox partner on the reduction potentials of the enzyme copper centers (2014) Journal of Biological Inorganic Chemistry, 19 (6), pp. 913-921.
Otrelo-Cardoso, A.R., Nair, R.R., Correia, M.A.S., Rivas, M.G., Santos-Silva, T. TupA: A tungstate binding protein in the periplasm of Desulfovibrio alaskensis G20 (2014) International Journal of Molecular Sciences, 15 (7), pp. 11783-11798.
Fliedel, C., Rosa, V., Santos, C.I.M., Gonzalez, P.J., Almeida, R.M., Gomes, C.S.B., Gomes, P.T., Lemos, M.A., Aullón, G., Welter, R., Aviles, T. Copper(ii) complexes of bis(aryl-imino)acenaphthene ligands: Synthesis, structure, DFT studies and evaluation in reverse ATRP of styrene (2014) Dalton Transactions, 43 (34), pp. 13041-13054.
2013
Marangon, J., Correia, H.D., Brondino, C.D., Moura, J.J.G., Romao, M.J., Gonzalez, P.J., Santos-Silva, T. Kinetic and structural studies of aldehyde oxidoreductase from Desulfovibrio gigas reveal a dithiolene-based chemistry for enzyme activation and inhibition by H2O2 (2013) PLoS ONE, 8 (12), art. no. e83234.
Thapper, A., Rizzi, A.C., Brondino, C.D., Wedd, A.G., Pais, R.J., Maiti, B.K., Moura, I., Pauleta, S.R., Moura, J.J.G. Copper-substituted forms of the wild type and C42A variant of rubredoxin (2013) Journal of Inorganic Biochemistry, 127, pp. 232-237.
Gonzalez, P.J., Rivas, M.G., Mota, C.S., Brondino, C.D., Moura, I., Moura, J.J.G. Periplasmic nitrate reductases and formate dehydrogenases: Biological control of the chemical properties of Mo and W for fine tuning of reactivity, substrate specificity and metabolic role (2013) Coordination Chemistry Reviews, 257 (2), pp. 315-331.
Cerqueira, N.M.F.S.A., Fernandes, P.A., Gonzalez, P.J., Moura, J.J.G., Ramos, M.J. The sulfur shift: An activation mechanism for periplasmic nitrate reductase and formate dehydrogenase (2013) Inorganic Chemistry, 52 (19), pp. 10766-10772.
2012
Neuman, N.I., Franco, V.G., Ferroni, F.M., Baggio, R., Passeggi, M.C.G., Rizzi, A.C., Brondino, C.D. Single crystal EPR of the mixed-ligand complex of copper(II) with l -glutamic acid and 1,10-phenanthroline: A study on the narrowing of the hyperfine structure by exchange (2012) Journal of Physical Chemistry A, 116 (50), pp. 12314-12320.
Ferroni, F.M., Guerrero, S.A., Rizzi, A.C., Brondino, C.D. Overexpression, purification, and biochemical and spectroscopic characterization of copper-containing nitrite reductase from Sinorhizobium meliloti 2011.Study of the interaction of the catalytic copper center with nitrite and NO (2012) Journal of Inorganic Biochemistry, 114, pp. 8-14.
Marangon, J., Paes De Sousa, P.M., Moura, I., Brondino, C.D., Moura, J.J.G., Gonzalez, P.J. Substrate-dependent modulation of the enzymatic catalytic activity: Reduction of nitrate, chlorate and perchlorate by respiratory nitrate reductase from Marinobacter hydrocarbonoclasticus 617 (2012) Biochimica et Biophysica Acta - Bioenergetics, 1817 (7), pp. 1072-1082.
2011
Mota, C.S., Rivas, M.G., Brondino, C.D., Moura, I., Moura, J.J.G., Gonzalez, P.J., Cerqueira, N.M.F.S.A. The mechanism of formate oxidation by metal-dependent formate dehydrogenases (2011) Journal of Biological Inorganic Chemistry, 16 (8), pp. 1255-1268.
Ferroni, F.M., Rivas, M.G., Rizzi, A.C., Lucca, M.E., Perotti, N.I., Brondino, C.D. Nitrate reduction associated with respiration in Sinorhizobium meliloti 2011 is performed by a membrane-bound molybdoenzyme (2011) BioMetals, 24 (5), pp. 891-902.
Mota, C.S., Valette, O., Gonzalez, P.J., Brondino, C.D., Moura, J.J.G., Moura, I., Dolla, A., Rivas, M.G. Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491 (2011) Journal of Bacteriology, 193 (12), pp. 2917-2923.
Silva, A.M.G., Leite, A., Gonzalez, P., Domingues, M.R.M., Gameiro, P., De Castro, B., Rangel, M. Use of a porphyrin platform and 3,4-HPO chelating units to synthesize ligands with N4 and O4 coordination sites (2011) Tetrahedron, 67 (40), pp. 7821-7828.
Coelho, C., Gonzalez, P.J., Moura, J.G., Moura, I., Trincao, J., Joao Romao, M. The crystal structure of cupriavidus necator nitrate reductase in oxidized and partially reduced states (2011) Journal of Molecular Biology, 408 (5), pp. 932-948.
2010
Nunez, C., Bastida, R., Macias, A., Valencia, L., Neuman, N.I., Rizzi, A.C., Brondino, C.D., Gonzalez, P.J., Capelo, J.L., Lodeiro, C. Structural, MALDI-TOF-MS, Magnetic and Spectroscopic Studies of New Dinuclear Copper(II), Cobalt(II) and Zinc(II) Complexes Containing a Biomimicking mu-OH bridge (2010) Dalton Transactions, 39 (48), pp. 11654-11663.
Neuman, N.I., Perec, M., Gonzalez, P.J., Passeggi, M.C.G., Rizzi, A.C., Brondino, C.D. Single crystal EPR study of the dinuclear Cu(II) complex [Cu(tda)(phen)]2·H2tda (tda = Thiodiacetate, phen = Phenanthroline): Influence of weak interdimeric magnetic interactions (2010) Journal of Physical Chemistry A, 114 (50), pp. 13069-13075.
Santos, H.M., Carreira, R., Diniz, M.S., Rivas, M.G., Lodeiro, C., Moura, J.J.G., Capelo, J.L. Ultrasonic multiprobe as a new tool to overcome the bottleneck of throughput in workflows for protein identification relaying on ultrasonic energy (2010) Talanta, 81 (1-2), pp. 55-62.